The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
ApplicationGenElute™ Plant Genomic DNA Miniprep Kit has been used:
- in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
- in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
- in genomic DNA isolation from leaves
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No