Dulbecco’s Modified Eagle’s Medium - low glucose With 1000 mg/L glucose, and sodium bicarbonate, without ?-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:

• living colon cancer SW620 cells[1]
• human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231[2]
• mammal and canine derived adipose-derived mesenchymal stem cells[2]