The REDExtract-N-Amp™ PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.
ApplicationREDExtract-N-Amp™ PCR Master Mix has been used:
- for the genotyping of fixed zebrafish embryo samples
- in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp™ Plant PCR Kit
- in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp™ seed PCR kit.
REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.
Features and Benefits
- A Gel-ready: No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
- Convenience: Hot-start feature in JumpStart™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
- Reproducibility: Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
- High Specificity & Yield: JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.