General description
Accutase® solution (in Dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion- coated plasticware. Accutase is preferred over trypsin for cell detachment as it helps to detach cells from culture vessels with less damage. Hence, Accutase is often used for the treatment of sensitive cells or for analyses of surface markers.

Application
Accutase® solution has been used for dissociation or digestion of nonadherent cells growing in an aggregated cluster, such as brain tumor cells,[1] induced pluripotent stem cells (IPSCs)[2] and glioblastoma cells[3] into single cells.

Suitable for use in preparation of single cell suspension for sequencing.
Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.

Features and Benefits
Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.