EVAGREEN 20x in water 1ml

Catalogue Number: BTM31000

Volume: 20 x 1ml

EvaGreen

EvaGreen dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.

The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

Features:

• Low PCR inhibition
  Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
• Highly Sensitive
  Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
• Nonmutagenic and noncytotoxic
  Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
• Compatible with fast PCR protocols
  Minimal interference to PCR makes it possible to significantly shorten the chain extension time
• Compatible with multiplex PCR
  No dye migration from amplicon to amplicon when used at the recommended concentration
• Unsurpassed thermal stability, chemical stability and photostability
  No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
• Spectrally similar to SYBR Green I
  Compatible with all major brand qPCR instruments and enzyme systems