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    3D Cell Culture - 3D in a fast and easy 2D workflow

    Thursday, June 13, 2019

    Greiner Bio-one has developed a system of 3D Cell Culture tools to open new dimensions and allow the processing of a 3D cell culture in a fast and easy 2D workflow.

    Magnetic levitation is an easy tool to create native tissue environments in vitro. In magnetic levitation the magnetized cells are levitated off the bottom by a magnet above the plate. By levitating cells off the plate bottom the magnetic forces work as an invisible scaffold that rapidly and gently aggregates cells and induces cell-cell interactions and ECM synthesis. The 3D culture is formed without any artificial substrate or specialized media or equipment and can be cultured long-term. The 3D culture is formed without any artificial substrate or specialized media or equipment and can be cultured long-term.The gentle nature of magnetic levitation allows cultures to acquire macroscale morphology that mostly resembles its tissue of origin. 3D cell cultures can be analyzed using common biological research techniques, such as immunohistochemical analysis and western blotting.

    The magnetic levitation has been successfully used to make 3D cultures with different cell types, including different cell lines, including stem cells and primary cells.

    In contrast to magnetic levitation, with magnetic 3D bioprinting, the magnetized cells in a plate are printed into spheroids by placing atop a drive of magnets. One magnet below each well utilizes mild magnetic forces to induce cell aggregation and print one spheroid at the bottom of each well anywhere within 15 minutes to a few hours. Afterwards the spheroids can be cultured long-term without the use of magnetic force. This system overcomes the limitations of other platforms by enabling rapid formation of spheroids, reproducible and scalable in size for high-throughput formats (96, 384 and 1536 well) and without limitation to cell types. The 3D printing method together with commercially available standardized biochemical assay methods to facilitate continuous assessment of cell viability and other functions, provides an ideal combination for high-throughput compound screening.

    The BiO Assay combines 3D cell culture environments with high-throughput and high-content testing to effectively predict in vivo response in vitro.

    In contrast to standard tissue culture surfaces which are optimised to enhance conditions for cell attachment, the cell-repellent surface has been developed to effectively prevent cell attachment. CELLSTAR® cell culture vessels with a cell-repellent surface reliably prevent cell attachment in suspension cultures of semiadherent and adherent cell lines where standard hydrophobic surfaces generally used for suspension culture are insufficient.


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