Amicon® Ultra-0.5 centrifugal filter devices for protein or DNA purification and concentration provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex samples . When used for DNA purification and concentration the Amicon® Ultra 0.5 device provides the best balance between recovery and spin time for double stranded DNA base pairs form 137 to 1150. The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent contamination of the membrane are also minimized by the vertical design, and a physical dead-stop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample is achieved by a convenient reverse spin step after collecting the filtrate. Advantages: • Sample concentration from 500µl down to 15µl • Concentration factor of 25 – 30x • High sample recovery – greater than 90% of dilute starting solution • Fast processing time of 10 – 30 minutes • Reverse spin capability provides consistent recoveries Applications: • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC • Desalting, buffer exchange, and protein dialysis Specifications: • Maximum initial sample volume – 500µl • Typical final concentration volume – 15–20µl • Recommended relative centrifugal force - 14,000 x g for concentration spin 1,000 x g for reverse spin • Maximum relative centrifugal force - 15,000 x g • Active membrane area - 1 cm² • Hold up volume – < 1µl